PIGY Rabbit Polyclonal Antibody

CAT#: TA306696

Rabbit Polyclonal PIG-Y Antibody

Product Images

Western blot analysis of PIG-Y in human spleen tissue lysate with PIG-Y antibody at (A) 1 and (B) 2 ug/ml.

• 

  Immunohistochemistry of PIG-Y in human spleen tissue with PIG-Y antibody at 2.5 ug/ml.

• 

  Immunofluorescence of PIGY in human spleen tissue with PIGY1 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, ICC: 2.5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: PIG-Y antibody was raised against a 16 amino acid peptide near the center of human PIG-Y.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: phosphatidylinositol glycan anchor biosynthesis class Y
• Database Link:
  NP_001036081
  Entrez Gene 84992 Human
  UniProt ID Q3MUY2
• Background: Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA. This antibody only detects isoform 1 of PIG-Y. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE.
• Synonyms: HPMRS6; PIG-Y

Reference Data

• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways