AP2M1 Chicken Polyclonal Antibody

CAT#: TA306777

Chicken Polyclonal AP2M1 Antibody

Product Images

Western blot analysis of AP2M1 in human kidney tissue lysate with AP2M1 antibody at (A) 1 and (B) 2 ug/ml.

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  Immunohistochemistry of AP2M1 in mouse kidney tissue with AP2M1 antibody at 2.5 ug/ml.

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  Immunofluorescence of AP2M1 in Mouse Kidney cells with AP2M1 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, ICC: 2.5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Chicken
• Isotype: IgY
• Clonality: Polyclonal
• Immunogen: AP2M1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human AP2M1.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: adaptor related protein complex 2 mu 1 subunit
• Database Link:
  NP_001020376
  Entrez Gene 11773 MouseEntrez Gene 116563 RatEntrez Gene 1173 Human
  UniProt ID Q96CW1
• Background: The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family. The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP2M1 has also been shown to associate with the HIV-1 protein Nef, suggesting that Nef may use AP-2 complex to enhance the rate of endocytosis of both CD4 and class I MHC. AP2M1 may also play an important role in regulating the intracellular trafficking and function of cytotoxic T-lymphocyte associated (CTLA)-4 protein. At least two isoforms of AP2M1 are known to exist.
• Synonyms: AP50; CLAPM1; mu2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Huntington's disease