RAG2 Rabbit Polyclonal Antibody

CAT#: TA307957

Rabbit polyclonal antibody to RAG2 (recombination activating gene 2)

Product Images

Sample (30 ug of whole cell lysate). A: Molt-4. 7.5% SDS PAGE. TA307957 diluted at 1:1000.

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  RAG2 antibody detects RAG2 protein by Western blot analysis. A. 50 ug Rat thymus lysate/extract. 7.5 % SDS-PAGE. RAG2 antibody (TA307957) dilution: 1:1000

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  Immunohistochemical analysis of paraffin-embedded DLD1 Xenograft, using RAG2 (TA307957) antibody at 1:100 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RAG2 (TA307957) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 271 and 527 of RAG2 (Uniprot ID#P55895)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 59 kDa
• Gene Name: recombination activating gene 2
• Database Link:
  NP_000527
  Entrez Gene 19374 MouseEntrez Gene 295953 RatEntrez Gene 5897 Human
  UniProt ID P55895
• Background: This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq]
• Synonyms: RAG-2
• Note: Seq homology of immunogen across species: Mouse (85%), Pig (90%), Rabbit (91%), Rat (86%), Bovine (91%)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Primary immunodeficiency