Cytokeratin 2 (KRT2) Rabbit Polyclonal Antibody

CAT#: TA307989

Rabbit Polyclonal antibody to Cytokeratin 2 (keratin 2)

Product Images

Sample (30 ug of whole cell lysate). A: A431

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  Immunohistochemical analysis of paraffin-embedded H661 xenograft, using KRT2 (TA307989) antibody at 1:100 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed A431, using Cytokeratin 2 (TA307989) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 143 and 529 of Cytokeratin 2 (Uniprot ID#P35908)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 65 kDa
• Gene Name: keratin 2
• Database Link:
  NP_000414
  Entrez Gene 444850 DogEntrez Gene 3849 Human
  UniProt ID P35908
• Background: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq]
• Synonyms: CK-2e; K2e; KRT2A; KRT2E; KRTE
• Note: Seq homology of immunogen across species: Dog (82%)