NDUFS2 Rabbit Polyclonal Antibody

CAT#: TA307995

Rabbit Polyclonal antibody to NDUFS2 (NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase))

Product Images

Sample (50 ug of whole cell lysate). A: Mouse brain. 10% SDS PAGE. TA307995 diluted at 1:10000.

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  Sample (30 ug of whole cell lysate). A: 293T. B: A431. C: JurKat. D: Raji. 10% SDS PAGE. TA307995 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded Colon ca, using NDUFS2 (TA307995) antibody at 1:250 dilution.

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  NDUFS2 antibody detects NDUFS2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30min. Green: NDUFS2 protein stained by NDUFS2 antibody (TA307995) diluted at 1:500. Blu

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:1000-1:10000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 200 and 430 of NDUFS2 (Uniprot ID#O75306)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53 kDa
• Gene Name: NADH:ubiquinone oxidoreductase core subunit S2
• Database Link:
  NP_004541
  Entrez Gene 226646 MouseEntrez Gene 4720 Human
  UniProt ID O75306
• Background: Mitochondrial complex I (NADH-ubiquinone reductase; EC 1.6.5.3) is the first multimeric complex of the respiratory chain that catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mammalian mitochondrial complex I is an assembly of at least 43 different subunits. Seven of the subunits are encoded by the mitochondrial genome; the remainder are the products of nuclear genes. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits, including NDUFS2. See NDUFS1 (MIM 157655). [supplied by OMIM]
• Synonyms: CI-49
• Note: Seq homology of immunogen across species: Chimpanzee (100%)

Reference Data

• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease