ARF5 Rabbit Polyclonal Antibody

CAT#: TA308198

Rabbit polyclonal antibody to ARF5 (ADP-ribosylation factor 5)

Product Images

Sample (30 ug of whole cell lysate). A:MOLT4. B:Raji. 15% SDS PAGE. TA308198 diluted at 1:1500

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  ARF5 antibody detects ARF5 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. ARF5 antibody (TA308198) dilution: 1:500.

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  Immunofluorescence analysis of methanol-fixed HeLa, using ARF5 (TA308198) antibody at 1:500 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region within amino acids 49 and 180 of ARF5
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21 kDa
• Gene Name: ADP ribosylation factor 5
• Database Link:
  NP_001653
  Entrez Gene 11844 MouseEntrez Gene 79117 RatEntrez Gene 381 Human
  UniProt ID P84085
• Background: ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns. [provided by RefSeq]
• Synonyms: ADP-ribosylation factor 5
• Note: Seq homology of immunogen across species: Mouse (100%), Rat (100%), Pig (100%), Chicken (93%), Sheep (100%), Bovine (100%)