SF3B3 Rabbit Polyclonal Antibody

CAT#: TA308399

Rabbit polyclonal antibody to SAP130 (splicing factor 3b, subunit 3, 130kDa)

Product Images

SAP130 antibody [C3], C-term detects SAP130 protein by western blot analysis. A. 30 ug HeLa whole cell extract. 5 % SDS-PAGE. SAP130 antibody [C3], C-term (TA308399) dilution: 1:10000

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  Immunohistochemical analysis of paraffin-embedded Saos-2 xenograft, using SF3B3 (TA308399) antibody at 1:100 dilution.

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  Immunofluorescence analysis of methanol-fixed HeLa, using SAP130 (TA308399) antibody at 1:500 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:20000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region within amino acids 1154 and 1217 of SAP130 (Uniprot ID#Q15393)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 136 kDa
• Gene Name: splicing factor 3b subunit 3
• Database Link:
  NP_036558
  Entrez Gene 101943 MouseEntrez Gene 292019 RatEntrez Gene 23450 Human
  UniProt ID Q15393
• Background: This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq]
• Synonyms: RSE1; SAP130; SF3b130; STAF130
• Note: Seq homology of immunogen across species: Mouse (100%), Rat (100%), Zebrafish (100%), Bovine (100%)

Reference Data

• Protein Pathways: Spliceosome