A RAF (ARAF) Rabbit Polyclonal Antibody

CAT#: TA308536

Rabbit Polyclonal antibody to ARAF (v-raf murine sarcoma 3611 viral oncogene homolog)

Product Images

Sample (30 ug of whole cell lysate). A: Hela. 7.5% SDS PAGE. TA308536 diluted at 1:1000.

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  Sample (30 ug of whole cell lysate). A:NIH-3T3. 7.5% SDS PAGE. TA308536 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ARAF (TA308536) antibody at 1:500 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed A431, using ARAF (TA308536) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 371 and 554 of A-RAF (Uniprot ID#P10398)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68 kDa
• Gene Name: A-Raf proto-oncogene, serine/threonine kinase
• Database Link:
  NP_001645
  Entrez Gene 11836 MouseEntrez Gene 64363 RatEntrez Gene 369 Human
  UniProt ID P10398
• Synonyms: A-RAF; ARAF1; PKS2; RAFA1
• Note: Seq homology of immunogen across species: Pig (99%), Rat (99%), Zebrafish (85%), Bovine (99%)

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Bladder cancer, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Glioma, Insulin signaling pathway, Long-term depression, Long-term potentiation, Melanoma, Natural killer cell mediated cytotoxicity, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Vascular smooth muscle contraction