PSME3 Rabbit Polyclonal Antibody

CAT#: TA308562

Rabbit Polyclonal antibody to PSME3 (proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki))

Product Images

Sample (30 ug of whole cell lysate). A: A549. B: H1299. C: HCT116. D: MCF-7. 12% SDS PAGE. TA308562 diluted at 1:2000.

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  Sample (30 ug of whole cell lysate). A: NIH-3T3. B: JC. C: BCL-1. 12% SDS PAGE. TA308562 diluted at 1:2000.

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  Sample (30 ug of whole cell lysate). A: PC-12. 12% SDS PAGE. TA308562 diluted at 1:2000.

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  Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using PSME3 (TA308562) antibody at 1:100 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PSME3 (TA308562) antibody at 1:500 dilution.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment contain a sequence corresponding to a region within amino acids 1 and 254 of PSME3
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30 kDa
• Gene Name: proteasome activator subunit 3
• Database Link:
  NP_005780
  Entrez Gene 19192 MouseEntrez Gene 10197 Human
  UniProt ID P61289
• Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Two transcript variants encoding different isoforms have been identified. [provided by RefSeq]
• Synonyms: HEL-S-283; Ki; PA28-gamma; PA28G; PA28gamma; REG-GAMMA
• Note: Seq homology of immunogen across species: Pig (100%)

Reference Data

• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Antigen processing and presentation, Proteasome