VCP Rabbit Polyclonal Antibody

CAT#: TA308570

Rabbit Polyclonal antibody to VCP (valosin-containing protein)

Product Images

VCP antibody detects VCP protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 7.5 % SDS-PAGE. VCP antibody (TA308570) dilution: 1:1000

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  Sample (50 ug of whole cell lysate). A: mouse brain. 7.5% SDS PAGE. TA308570 diluted at 1:5000.

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  Sample (30 ug of whole cell lysate). A: Hela. B: Hep G2. C: Molt-4. D: Raji. 7.5% SDS PAGE. TA308570 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using VCP (p97) (TA308570) antibody at 1:500 dilution.

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  VCP antibody [N1N2], N-term detects VCP protein at nucleus and cytosol on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. VCP antibody [N1N2], N-term (TA308570) dilution: 1:500.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using VCP (p97) (TA308570) antibody at 1:200 dilution.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:1000-1:10000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 206 of VCP (Uniprot ID#P55072)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 89 kDa
• Gene Name: valosin containing protein
• Database Link:
  NP_009057
  Entrez Gene 269523 MouseEntrez Gene 7415 Human
  UniProt ID P55072
• Background: The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]
• Synonyms: ALS14; CMT2Y; HEL-220; HEL-S-70; IBMPFD; IBMPFD1; p97; TERA
• Note: Seq homology of immunogen across species: Chicken (99%), Pig (99%), Xenopus laevis (98%), Zebrafish (97%), Bovine (99%), Xenopus tropicalis (99%)