DUSP3 Rabbit Polyclonal Antibody

CAT#: TA308662

Rabbit Polyclonal antibody to DUSP3 (dual specificity phosphatase 3)

Product Images

Sample (50 ug of whole cell lysate). A: mouse stomach. 12% SDS PAGE. TA308662 diluted at 1:1000.

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  DUSP3 antibody detects DUSP3 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 15 % SDS-PAGE, and blotted with DUSP3 antibody (TA308662) diluted by 1:1000

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  Immunohistochemical analysis of paraffin-embedded N87 xenograft, using DUSP3 (TA308662) antibody at 1:500 dilution.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 185 of DUSP3 (Uniprot ID#P51452)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20 kDa
• Gene Name: dual specificity phosphatase 3
• Database Link:
  NP_004081
  Entrez Gene 72349 MouseEntrez Gene 1845 Human
  UniProt ID P51452
• Background: The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. [provided by RefSeq]
• Synonyms: VHR
• Note: Seq homology of immunogen across species: Rhesus Monkey (100%)

Reference Data

• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: MAPK signaling pathway