ME1 Rabbit Polyclonal Antibody

CAT#: TA308668

Rabbit polyclonal antibody to ME1 (malic enzyme 1, NADP(+)-dependent, cytosolic)

Product Images

Sample (30 ug of whole cell lysate). A: NIH-3T3. B: JC. C: BCL-1. 7.5% SDS PAGE. TA308668 diluted at 1:1000.

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  Sample (30 ug of whole cell lysate). A: PC-12. 7.5% SDS PAGE. TA308668 diluted at 1:1000.

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  Sample (30 ug of whole cell lysate). A: A431. 7.5% SDS PAGE. TA308668 diluted at 1:1000.

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  ME1 antibody detects ME1 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. ME1 antibody (TA308668) dilution: 1:500.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ME1 (TA308668) antibody at 1:200 dilution.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 161 and 519 of (Uniprot ID#P48163)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 64 kDa
• Gene Name: malic enzyme 1
• Database Link:
  NP_002386
  Entrez Gene 4199 Human
  UniProt ID P48163
• Background: This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq]
• Synonyms: HUMNDME; MES

Reference Data

• Protein Pathways: Metabolic pathways, PPAR signaling pathway, Pyruvate metabolism