XPB (ERCC3) Rabbit Polyclonal Antibody

CAT#: TA308754

Rabbit polyclonal antibody to XPB (excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing))

Product Images

Sample (30 ug of whole cell lysate). A: 293T. B: A431. C: H1299. 7.5% SDS PAGE. TA308754 diluted at 1:1000.

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  XPB antibody detects XPB protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cel

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using XPB (TA308754) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 521 and 742 of XPB (Uniprot ID#P19447)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 89 kDa
• Gene Name: ERCC excision repair 3, TFIIH core complex helicase subunit
• Database Link:
  NP_000113
  Entrez Gene 2071 Human
• Background: ERCC3 is an ATP-dependent DNA helicase that functions in nucleotide excision repair and complements xeroderma pigmentosum group B mutations. It also is the 89 kDa subunit of basal transcription factor 2 (TFIIH) and thus functions in class II transcription. [provided by RefSeq]
• Synonyms: BTF2; GTF2H; RAD25; TFIIH; TTD2; XPB
• Note: Seq homology of immunogen across species: Rhesus Monkey (100%), Bovine (100%)

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Nucleotide excision repair