XRCC4 Rabbit Polyclonal Antibody

CAT#: TA308834

Rabbit Polyclonal antibody to XRCC4 (X-ray repair complementing defective repair in Chinese hamster cells 4)

Product Images

Sample (30 ug of whole cell lysate). A: Jurkat. B: K562. C: HL-60. 10% SDS PAGE. TA308834 diluted at 1:1000.

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  XRCC4 antibody detects XRCC4 protein at nucleus on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. XRCC4 antibody (TA308834) dilution: 1:500.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using XRCC4 (TA308834) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region within amino acids 216 and 309 of XRCC4 (Uniprot ID#Q13426)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38 kDa
• Gene Name: X-ray repair complementing defective repair in Chinese hamster cells 4
• Database Link:
  NP_071801
  Entrez Gene 108138 MouseEntrez Gene 309995 RatEntrez Gene 7518 Human
  UniProt ID Q13426
• Background: The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors. This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. Alternative transcription initiation and alternative splicing generates several transcript variants. [provided by RefSeq]
• Synonyms: SSMED
• Note: Seq homology of immunogen across species: Mouse (86%), Rat (86%), Bovine (93%)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Non-homologous end-joining