Dnm1 Sheep Polyclonal Antibody

CAT#: TA309114

Sheep Anti-Dynamin (Ser774) Antibody (Phospho-Specific)

Product Images

Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser774 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser774 dynamin. The immunolabeling is completely eliminated by treatment with λ-Ptase.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:100, IHC: 1:1000, IF: 1:1000
• Reactivities: Rat
• Modifications: Phospho-specific
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 conjugated to KLH
• Formulation: 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 95 kDa
• Gene Name: dynamin 1
• Database Link:
  NP_542420
  Entrez Gene 140694 Rat
  UniProt ID P21575
• Synonyms: DNM