BRCA1 Mouse Monoclonal Antibody [Clone ID: KEN]

CAT#: TA309591

Mouse Monoclonal BRCA1 Antibody (KEN)

Product Images

Immunocytochemistry/Immunofluorescence: BRCA1 Antibody (KEN) TA309591 - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-BRCA1 (KEN) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Western Blot: BRCA1 Antibody (KEN) TA309591 - Detection of BRCA1.

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  Western Blot: BRCA1 Antibody (KEN) TA309591 - Detection of BRCA1 in U2OS whole cell lysate using NB 100-599. 1 minute ECL detection.

Specifications

Product Data

• Clone Name: KEN
• Applications: WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence: 1:100, Immunoprecipitation: 1:10-1:500, Western Blot: 2-4 ug/ml
• Reactivities: Human
• Host: Mouse
• Isotype: IgG3, kappa
• Clonality: Monoclonal
• Immunogen: Human BRCA1 (residues 1314-1864).
• Formulation: Tris-glycine, 150mM NaCl pH 7.5 and 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: BRCA1, DNA repair associated
• Database Link:
  NP_009225
  Entrez Gene 672 Human
  UniProt ID P38398
• Synonyms: BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4; PPP1R53; PSCP; RNF53

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Ubiquitin mediated proteolysis