LY75 (LY75-CD302) Rabbit Monoclonal Antibody [Clone ID: EPR5233]

CAT#: TA310261

Rabbit Monoclonal antibody against CD205

Product Images

Western blot - Anti-LY75 antibody [EPR5233]; All lanes : Anti-LY75 antibody [EPR5233] at 1/1000 dilution.Lane 1 : Human thymus lysate.Lane 2 : Human lymph node lysate.Lane 3 : Human tonsil lysate.Lane 4 : Mouse thymus lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 198 kDa.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75 antibody [EPR5233]; TA310261, at 1/250 dilution, staining LY75 in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-LY75 antibody [EPR5233] (TA310261); TA310261 showing negative staining in Normal kidney tissue.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-LY75 antibody [EPR5233] (TA310261); TA310261 showing negative staining in Normal brain tissue.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-LY75 antibody [EPR5233] (TA310261); TA310261 showing positive staining in Normal thymus tissue.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-LY75 antibody [EPR5233] (TA310261); TA310261 showing positive staining in Normal spleen tissue.

• Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-LY75 antibody [EPR5233] (TA310261); TA310261 showing negative staining in Skeletal muscle tissue.

• Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-LY75 antibody [EPR5233] (TA310261); TA310261 showing positive staining in Normal colon lymphocytes tissue.

• Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-LY75 antibody [EPR5233] (TA310261); TA310261 showing negative staining in Normal lung tissue.

• Other-Anti-LY75 antibody [EPR5233] (TA310261); Equilibrium disassociation constant (KD).

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Specifications

Product Data

• Clone Name: EPR5233
• Applications: Assay, IHC, WB
• Recommended Dilution: WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; ICC: 1:100 - 1:250
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponding to residues in the intracellular domain of human CD205 was used as an immunogen.
• Formulation: pH: 7.40
  Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol, 0.05% BSA
• Purification: Tissue culture supernatant
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 198 kDa
• Gene Name: LY75-CD302 readthrough
• Database Link:
  NP_001185688
  Entrez Gene 100526664 Human
  UniProt ID O60449
• Background: CD205 is a type I C-type lectin-like molecule that acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment (1, 2). It functions to deliver antigen to dendritic cells for presentation on MHC class II and cross-presentation on MHC class I. Thus, CD205 plays an important role in CD4 and CD8 T cell tolerance induction to self-antigen both in the periphery and in the thymus. It is expressed at high levels on cortical thymic epithelium, thymic medullary DCs (CD11c+ CD8+), and subsets of peripheral DCs (CD11c+ CD8+ splenic/lymph node DCs, dermal/interstitial DCs, and Langerhans cells) (2). In addition, CD205 causes reduced proliferation of B-lymphocytes (1).
• Note: Does not react with Rat