HSP90AB1 Rabbit Polyclonal Antibody

CAT#: TA312343

Rabbit polyclonal HSP90B (Ser254) antibody(Phospho-specific)

Product Images

Western blot analysis of extracts from Hela cells treated with TNF-a (10ng/ml, 30mins), using HSP90B (phospho-Ser254) antibody.The lane on the right is treated with the synthesized peptide.

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  Western blot analysis of extracts from JK cells (Lane 2) and K562 cells (Lane 3), using HSP90B (Phospho-Ser254) Antibody. The lane on the left is treated with synthesized peptide.

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP90B (phospho-Ser254) antibody.The picture on the right is treated with the synthesized peptide.

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  Immunofluorescence analysis of HeLa cells, treated with TNF-a (20nM, 15mins), using HSP90B (phospho-Ser254) antibody (#11313).The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500~3000, IHC: 1:50~100, IF: 1:100~1:500, ELISA: 1:40000
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human HSP90B around the phosphorylation site of serine 254 (V-G-SP-D-E).
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: heat shock protein 90kDa alpha family class B member 1
• Database Link:
  NP_031381
  Entrez Gene 15516 MouseEntrez Gene 301252 RatEntrez Gene 3326 Human
  UniProt ID P08238
• Synonyms: D6S182; HSP84; HSP90B; HSPC2; HSPCB
• Note: HSP90B (phospho-Ser254) antibody detects endogenous levels of HSP90B only when phosphorylated at serine 254.

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Antigen processing and presentation, NOD-like receptor signaling pathway, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer