EGFR Rabbit Polyclonal Antibody

CAT#: TA312536

Rabbit polyclonal EGFR (Thr678) antibody(Phospho-specific)

Product Images

Western blot analysis of extracts from cos7 cells treated with EGF (200ng/ml, 30mins), using EGFR (phospho-Thr678) antibody.The lane on the right is treated with the synthesized peptide.

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  Immunofluorescence analysis of HuvEc cells, using EGFR (phospho-Thr678) antibody.The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:300~1:3000, IF: 1:100~1:500, ELISA: 1:40000
• Reactivities: Human, Mouse
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of threonine 678 (K-R-TP-L-R).
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: epidermal growth factor receptor
• Database Link:
  NP_005219
  Entrez Gene 13649 MouseEntrez Gene 1956 Human
  UniProt ID P00533
• Synonyms: ERBB; ERBB1; HER1; mENA; NISBD2; PIG61
• Note: EGFR (phospho-Thr678) antibody detects endogenous levels of EGFR only when phosphorylated at threonine 678.

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Secreted Protein, Stem cell relevant signaling - JAK/STAT signaling pathway, Transmembrane
• Protein Pathways: Adherens junction, Bladder cancer, Calcium signaling pathway, Colorectal cancer, Cytokine-cytokine receptor interaction, Dorso-ventral axis formation, Endocytosis, Endometrial cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton