NF2 Rabbit Polyclonal Antibody

CAT#: TA319136

Rabbit polyclonal NF2 phospho S518 antibody

Product Images

Phospho-specific antibody to NF2 (Merlin) at pS518 was used at a 1:1000 dilution to detect NF2 by WB. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2, 3 and 4 respectively) for 45'. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:1,200,000, WB: 1:500- 1:2,000, IHC: User Optimized
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Human NF2 (Merlin) phospho-peptide corresponding to a region of the human protein surrounding S518 and conjugated to Keyhole Limpet Hemocyanin (KLH).
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: neurofibromin 2 (merlin)
• Database Link:
  NP_000259
  Entrez Gene 18016 MouseEntrez Gene 4771 Human
  UniProt ID P35240
• Synonyms: ACN; BANF; SCH
• Note: The recently isolated neurofibromatosis 2 tumor suppressor gene encodes a 595 amino acid protein (Merlin). The protein product Merlin, named for its relatedness to the ezrin, radixin and moesin (ERM) family of proteins, is a tumor suppressor whose absence results in the occurrence of multiple tumors of the nervous system, particularly schwannomas and meningiomas. Merlin's similarity to ERM’s suggests that it might share functions, acting as a link between cytoskeletal components and the cell membrane. The NF2 protein is highly expressed in human fibroblasts and is detected as a single band of about 75kDa. This antibody is specific for the phosphorylated form of human NF2 (Merlin).

Reference Data

• Protein Families: Druggable Genome