Il17a Rabbit Polyclonal Antibody

CAT#: TA319352

Rabbit polyclonal anti-IL-17A antibody

Product Images

Western blot using anti-IL-17A antibody shows detection of rat recombinant IL-17A protein (lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight™ 649 conjugated Gt-a-Rabbit IgG (p/n 611-143-122). Molecular weight estimation was made by comparison to prestained MW markers indicated at the left. Other detection systems will yield similar results.

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  WB using anti-IL-17A antibody shows detection of mouse recombinant IL-17A protein (arrowhead, lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight™ 649 conjugated Gt-a-Rabbit IgG (p/n 611-143-122). Molecular weight estimation was made by comparison to prestained MW markers indicated at the left. Other detection systems will yield similar results.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:1,000 - 1:5,000, WB: 1:1,000
• Reactivities: Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant rat IL17-A protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: interleukin 17A
• Database Link:
  NP_001100367
  Entrez Gene 16171 MouseEntrez Gene 301289 Rat
• Synonyms: CTLA-8; CTLA8; IL-17; IL-17A; IL17
• Note: Rat IL17-A (also known as Interleukin-17, Cytotoxic T-lymphocyte-associated antigen 8 and CTLA-8) is a proinflammatory cytokine member of a six-species family of proteins (IL-17A-17F). Rat IL-17A protein is a homodimer consisting of two 134 amino acids peptides. IL-17A is secreted mainly by activated CD4+ and CD8+ T lymphocytes and acts through its receptor, IL-17R, to induce the expression of many mediators of inflammation, most strikingly, those that are involved in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been associated with several conditions, including rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer and multiple sclerosis. There is 58% identity between the amino acid sequence of human and rat IL-17A.