Apg3 (ATG3) Rabbit Polyclonal Antibody

CAT#: TA320002

Rabbit Polyclonal ATG3 Antibody

Product Images

Western blot analysis of ATG3 in Mouse kidney tissue Lysate with ATG3 antibody at (A) 1 and (B) 2 ug/mL.

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  Immunohistochemistry of ATG3 in human kidney tissue with ATG3 antibody at 5 ug/mL.

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  Immunofluorescence of ATG3 in human kidney with ATG3 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, ICC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: ATG3 antibody was raised against an 18 amino acid synthetic peptide near the center of human ATG3.
• Formulation: ATG3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: ATG3 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: autophagy related 3
• Database Link:
  NP_071933
  Entrez Gene 67841 MouseEntrez Gene 171415 RatEntrez Gene 64422 Human
  UniProt ID Q9NT62
• Background: ATG3 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG3 (APG3) is a widely expressed conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. It functions as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, which is critical for autophagy.
• Synonyms: APG3; APG3-LIKE; APG3L; PC3-96

Reference Data

• Protein Pathways: Regulation of autophagy