Prdm1 Rat Monoclonal Antibody [Clone ID: 6D3]

CAT#: TA320447

Rat Anti-Human/Mouse Blimp1 Purified (25 ug)

Product Images

Immunoblotting of reduced cell lysates prepared from A20 cell line using 5 ug/mL of Anti-Human/Mouse Blimp1 Purified. Bands were visualized using Anti-Rat IgG HRP (Left).

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  Immunohistochemistry on formalin-fixed paraffin embedded human tonsil tissue with citrate buffer antigen retrieval, using 10 ug/ml of Anti-Human/Mouse Blimp1 Purified or 10 ug/ml of Rat IgG2a kappa Isotype Control Purified followed by Anti-Rat IgG Biotin, and DAB visualization (Center, right).

Specifications

Product Data

• Clone Name: 6D3
• Applications: IHC, WB
• Recommended Dilution: IHC, WB
• Reactivities: Mouse
• Host: Rat
• Clonality: Monoclonal
• Formulation: Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: PR domain containing 1, with ZNF domain
• Database Link:
  NP_031574
  Entrez Gene 12142 Mouse
  UniProt ID Q60636
• Background: This 6D3 monoclonal antibody reacts with B lymphocyte-induced maturation protein-1 (Blimp-1), a transcriptional repressor expressed in B and T cells. This approximately 90-kDa protein contains five consecutive Kruppel-like zinc finger domains by which it binds DNA. In B cells, Blimp-1 promotes terminal differentiation into antibody-secreting plasma cells during a primary immune response. In addition, Blimp-1 has been implicated in germinal center formation in response to T-dependent and T-independent antigens, as well as in some diffuse large B cell lymphomas. Expressed in CD4+ and CD8+ effector/memory T cells and FoxP3+ regulatory T cells, reports have suggested possible roles for Blimp-1 in T cell homeostasis and suppression of IL-2 production. Moreover, by suppressing Th1 differentiation, Blimp-1 plays a role in T helper cell differentiation. Finally, Blimp-1 has been demonstrated to play a significant role in the terminal differentiation of effector and effector memory CD8+ T cells during viral infection.
• Synonyms: BLIMP-1; BLIMP1; MGC118922; MGC118923; MGC118924; MGC118925; PRDI-BF1