Spn Rat Monoclonal Antibody [Clone ID: eBioR2/60]

CAT#: TA320466

Rat Anti-Mouse CD43 Purified (100 ug)

Product Images

Staining of C57Bl/6 splenocytes with 0.25 ug of Rat IgM Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD43 Purified (filled histogram) followed by Anti-Rat IgM PE. Cells in the lymphocyte gate were used for analysis.

Specifications

Product Data

• Clone Name: eBioR2/60
• Applications: FC
• Recommended Dilution: Flow, IP, WB
• Reactivities: Mouse
• Host: Rat
• Clonality: Monoclonal
• Formulation: Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: sialophorin
• Database Link:
  NP_033285
  Entrez Gene 20737 Mouse
  UniProt ID P15702
• Background: The eBioR2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in BM, thymocytes and mature T cells, NK cells, myeloid lineage cells and platelets. CD43 is reported to bind to CD54 and play a role in adhesion and B cell survival. While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects the expression of both isoforms. It has been demonstrated that R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. However, the clones S7 and 1B11 stain only CD4+ and CD8+, or CD19+ splenocytes, respectively. This suggests that S7 and 1B11 recognize distinct epitopes which are differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43.
• Synonyms: CD43; Galactoglycoprotein; GALGP; GPL115; leukosialin; LSN; Sialophorin