GAB1 Rabbit Polyclonal Antibody

CAT#: TA321427

Anti-GAB1 (Phospho-Tyr627) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 77 kDa. Positive control: 293 cells treated with EGF lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane 1: 293 cells untreated with EGF lysate Lane 2: 293 cells treated with EGF lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Cell membrane. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using GAB1 (Phospho-Tyr627) antibody at dilution 1/100. (Original magnification: ×200)

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:500-1000, IF: 1:100-200
• Reactivities: Human, Mouse
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of tyrosine 627 (V-E-Y(p)-L-D) derived from Human Gab1.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 77 kDa
• Gene Name: GRB2 associated binding protein 1
• Database Link:
  NP_002030
  Entrez Gene 14388 MouseEntrez Gene 2549 Human
  UniProt ID Q13480
• Background: The protein encoded by Gab1 is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene.
• Synonyms: GRB2-associated binding protein 1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: ErbB signaling pathway, Neurotrophin signaling pathway, Renal cell carcinoma