Histone H2A.X (H2AFX) Rabbit Polyclonal Antibody

CAT#: TA321434

Anti-H2AFX (Phospho-Ser139) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 15 kDa. Positive control: HT29 cells untreated or treated with UV lysate. Recommended dilution: 1/ 500-1000. (Gel: 12%SDS-PAGE Lane 1: HT29 cells untreated with UV lysate Lane 2: HT29 cells treated with UV lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Nucleus. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using H2AFX (Phospho-Ser139) antibody at dilution 1/100. (Original magnification:×200)

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:500-1000, IF: 1:100-200
• Reactivities: Human
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of serine 139 (Q-A-S(p)-Q-E) derived from Human Histone H2A.X.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 15 kDa
• Gene Name: H2A histone family member X
• Database Link:
  NP_002096
  Entrez Gene 3014 Human
  UniProt ID P16104
• Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
• Synonyms: H2A; H2A.X; H2AX; X

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Systemic lupus erythematosus