MAP3K4 Rabbit Polyclonal Antibody

CAT#: TA322543

Anti-MAP3K4 Rabbit Polyclonal Antibody

Product Images

Immunohistochemistry of paraffin-embedded Human breast cancer tissue using TA322543 (MAP3K4 Antibody) at dilution 1/20 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human breast cancer tissue using TA322543 (MAP3K4 Antibody) at dilution 1/20, treated with synthetic peptide. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA322543 (MAP3K4 Antibody) at dilution 1/20 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA322543 (MAP3K4 Antibody) at dilution 1/20, treated with synthetic peptide. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: IHC: 25-100
  Positive control: Human breast cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region derived from 1282-1286 amino acids of human mitogen-activated protein kinase kinase kinase 4
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: mitogen-activated protein kinase kinase kinase 4
• Database Link:
  NP_005913
  Entrez Gene 26407 MouseEntrez Gene 308106 RatEntrez Gene 4216 Human
  UniProt ID Q9Y6R4
• Background: The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK); which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation; the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock; UV irradiation; wound stress; and inflammatory factors. This gene encodes a MAPKKK; the MEKK4 protein; also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways; but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1; MAPKKs that activate CSBP2 and JNK; respectively but cannot phosphorylate PRKMK1; an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway; and a minor mediator of the JNK pathway. Two alternatively spliced transcripts encoding distinct isoforms have been described.
• Synonyms: MAPKKK4; MEKK 4; MEKK4; MTK1; PRO0412

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: GnRH signaling pathway, MAPK signaling pathway