Caspase 3 (CASP3) Rabbit Polyclonal Antibody

CAT#: TA322764

Anti-CASP3 Rabbit Polyclonal Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane: THP-1 apoptotic cells after 48 hours of starvation Primary antibody: TA322764 (CASP3 Antibody) at dilution 1/400 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 7 seconds

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA322764 (CASP3 Antibody) at dilution 1/60 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA322764 (CASP3 Antibody) at dilution 1/60, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using TA322764 (CASP3 Antibody) at dilution 1/60 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using TA322764 (CASP3 Antibody) at dilution 1/60, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: THP-1 apoptotic cells after 48 hours of starvation
  IHC: 50-200
  Positive control: Human tonsil
  Predicted cell location: Cytoplasm and Nucleus
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to a region derived from 1-269 amino acids of human caspase 3, apoptosis-related cysteine peptidase
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32 kDa
• Gene Name: caspase 3
• Database Link:
  NP_004337
  Entrez Gene 12367 MouseEntrez Gene 25402 RatEntrez Gene 836 Human
  UniProt ID P42574
• Background: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits; large and small; that dimerize to form the active enzyme. This protein cleaves and activates caspases 6; 7 and 9; and the protein itself is processed by caspases 8; 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein; which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
• Synonyms: CPP32; CPP32B; SCA-1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protease
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, Huntington's disease, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, p53 signaling pathway, Parkinson's disease, Pathways in cancer, Viral myocarditis