Cullin 2 (CUL2) Rabbit Polyclonal Antibody

CAT#: TA322839

Anti-CUL2 Rabbit Polyclonal Antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 40 μg Lane: PC3 cells Primary antibody: TA322839 (CUL2 Antibody) at dilution 1/440 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 3 minutes

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 500-2000
  WB positive control: PC3 cells
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region derived from 214-228 amino acids of Human cullin 2
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 87 kDa
• Gene Name: cullin 2
• Database Link:
  NP_003582
  Entrez Gene 71745 MouseEntrez Gene 361258 RatEntrez Gene 8453 Human
  UniProt ID Q13617
• Background: Human CUL2 is an ortholog of nematode cul2. Michel and Xiong (1998) identified human CUL2 cDNAs and reported that the predicted protein is 745 amino acids long.Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes; which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 By similarity. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
• Synonyms: MGC131970

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Pathways in cancer, Renal cell carcinoma, Ubiquitin mediated proteolysis