PLCG 2 (PLCG2) Rabbit Polyclonal Antibody

CAT#: TA322975

Anti-PLCG2 Rabbit Polyclonal Antibody

Product Images

Predicted band size: 150 kDa. Positive control: Ramos cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500-2000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to N terminal 300 amino acids of human phospholipase C, gamma 2 (phosphatidylinositol-specific)
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 150 kDa
• Gene Name: phospholipase C gamma 2
• Database Link:
  NP_002652
  Entrez Gene 29337 RatEntrez Gene 234779 MouseEntrez Gene 5336 Human
  UniProt ID P16885
• Background: Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). At least four families of PLCs have been identified: PLC?, PLC?, PLC? and PLC?. The PLC? subfamily includes four members, PLC?1-4. All four members of the subfamily are activated by ?- or ?-?-subunits of the heterotrimeric G-proteins.Phosphorylation is one of the key mechanisms that regulates the activity of PLC. Phosphorylation of Ser1105 by PKA or PKC inhibits PLC?3 activity. Ser537 of PLC?3 is phosphorylated by CaMKII, and this phosphorylation may contribute to the basal activity of PLC?3. PLC? is activated by both receptor and nonreceptor tyrosine kinases.PLC? forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLC? at Tyr771, 783 and 1245. Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLC?1.PLC?2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197 and 1217 is correlated with PLC?2 activity.
• Synonyms: APLAID; FCAS3; PLC-gamma-2; PLC-IV

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: B cell receptor signaling pathway, Calcium signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glioma, Inositol phosphate metabolism, Leukocyte transendothelial migration, Metabolic pathways, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Phosphatidylinositol signaling system, VEGF signaling pathway, Vibrio cholerae infection