PLCG 2 (PLCG2) Rabbit Polyclonal Antibody

CAT#: TA322976

Anti-PLCG2 (Phospho-Tyr753) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 148 kDa. Positive control: HepG2 cells untreated or treated with EGF lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane 1: HepG2 cells untreated with EGF lysate Lane 2: HepG2 cells treated with EGF lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Cytoplasm. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using PLCG2 (Phospho-Tyr753) antibody at dilution 1/100. (Original magnification:×200)

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:500-1000, IF: 1:100-200
• Reactivities: Human, Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of tyrosine 753 (S-L-Y(p)-D-V) derived from Human PLC?2.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 148 kDa
• Gene Name: phospholipase C gamma 2
• Database Link:
  NP_002652
  Entrez Gene 29337 RatEntrez Gene 234779 MouseEntrez Gene 5336 Human
  UniProt ID P16885
• Background: The production of the second messenger molecules diacylglycerol. (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
• Synonyms: APLAID; FCAS3; PLC-gamma-2; PLC-IV

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: B cell receptor signaling pathway, Calcium signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glioma, Inositol phosphate metabolism, Leukocyte transendothelial migration, Metabolic pathways, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Phosphatidylinositol signaling system, VEGF signaling pathway, Vibrio cholerae infection