HDAC5 Rabbit Polyclonal Antibody

CAT#: TA323445

Anti-HDAC5 Rabbit Polyclonal Antibody

Product Images

Predicted band size: 122 kDa. Positive control: 293 and HepG2 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane 1, 2: 293 and HepG2 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Nucleus; Cytoplasm. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using HDAC5 (Ab-498) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500-1000, IHC: 1:50-100
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around aa.496~500 (T-Q-S-S-P) derived from Human HDAC5/7.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 122 kDa
• Gene Name: histone deacetylase 5
• Database Link:
  NP_001015053
  Entrez Gene 10014 Human
  UniProt ID Q9UQL6
• Background: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by HDAC5 belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.
• Synonyms: HD5; NY-CO-9

Reference Data

• Protein Families: Druggable Genome, Transcription Factors