RPS6 Rabbit Polyclonal Antibody

CAT#: TA323451

Anti-RPS6 Rabbit Polyclonal Antibody

Product Images

Predicted band size: 29 kDa. Positive control: HepG2 and 293 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane 1, 2: HepG2 and 293 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Cytoplasm. Positive control: Human lung carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human lung carcinoma tissue using RPS6 (Ab-235) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)

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  Predicted cell location: Cytoplasm. Positive control: MCF7 cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed MCF7 cells using RPS6 antibody at dilution 1/100. (Original magnification: ×200)

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around aa. 233~237 (R-L-S-S-L) derived from Human S6 Ribosomal Protein.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29 kDa
• Gene Name: ribosomal protein S6
• Database Link:
  NP_001001
  Entrez Gene 20104 MouseEntrez Gene 29304 RatEntrez Gene 6194 Human
  UniProt ID P62753
• Background: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
• Synonyms: S6

Reference Data

• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway, Ribosome