Sumo 3 (SUMO3) Rabbit Polyclonal Antibody

CAT#: TA324505

Rabbit polyclonal Pan SUMO Antibody

Product Images

SUMO3 Antibody (Cat. #TA324505) western blot analysis in 293 cell line and rat liver tissue lysates (35ug/lane).This demonstrates the SUMO3 antibody detected the SUMO3 protein (arrow).

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

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  IF image of Hela cell stained with Pan SUMO Antibody (Cat#TA324505).Hela cells were incubated with Pan SUMO primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue).Pan SUMO immunoreactivity is localized to Nucleus significantly.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IF: 1:10~50, IHC: 1:50~100
• Reactivities: Human, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This Pan SUMO antibody recognizes all 3 SUMO isoforms, including human SUMO1, SUMO2 and SUMO3. This antibody is generated from rabbits immunized with a recombinant protein encoding full length human SUMO3.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 11637 Da
• Gene Name: small ubiquitin-like modifier 3
• Database Link:
  NP_008867
  Entrez Gene 499417 RatEntrez Gene 6612 Human
  UniProt ID P55854
• Synonyms: SMT3A; Smt3B; SMT3H1; SUMO-3

Reference Data

• Protein Families: Druggable Genome