Telomerase reverse transcriptase (TERT) Rabbit Polyclonal Antibody

CAT#: TA324535

Rabbit polyclonal TERT Antibody (Center)

Product Images

Western blot analysis of anti-TERT Antibody (Center) ([RB14069]) in Jurkat cell line lysates (35ug/lane). TERT (arrow) was detected using the purified Pab.

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  IF image of Hela cell stained with TERT Antibody (Center) (Cat#TA324535).Hela cells were incubated with TERT primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). TERT immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.

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  IF image of SY5Y cells stained with TERT (Center) antibody. SY5Y cells were incubated with TA324535 TERT (Center) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). TERT immunoreactivity is localized to the nucleus of SY5Y cells.

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  TERT Antibody (Center) (Cat. #TA324535) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, WB
• Recommended Dilution: WB: 1:1000, FC: 1:10~50, IF: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This TERT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 627-656 amino acids from the Central region of human TERT.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 126997 Da
• Gene Name: telomerase reverse transcriptase
• Database Link:
  NP_937986
  Entrez Gene 7015 Human
  UniProt ID O14746
• Synonyms: EST2; hEST2; TCS1; telomerase catalytic subunit; telomerase reverse transcriptase; TP2; TRT

Reference Data

• Protein Families: Druggable Genome