MAP1LC3A Rabbit Polyclonal Antibody

CAT#: TA324626

Rabbit polyclonal Cleaved LC3A Antibody

Product Images

Western blot analysis of anti-cleaved-LC3 (APG8a) Pab (Cat. #TA324626) in mouse brain tissue lysate. Cleaved-LC3 (APG8a) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

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  5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in 4% of paraformaldehyde. Treatment with Cat# TA324626 antibody at dilution 1:100. Data courtesy of Jianhui Zhu, MD, PhD & Charleen T. Chu, MD, PhD, University of Pittsburgh School of Medicine.

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  IF image of U251 cells stained with cleaved LC3A antibody. U251 cells were treated with Chloroquine, then incubated with TA324626 cleaved LC3A primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

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  IF image of U251 cells stained with cleaved LC3A antibody. U251 cells were incubated with TA324626 cleaved LC3A primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: IF: 1:100, WB: 1:1000, IHC: 1:10~50, ICC: 1:10~50
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This Cleaved LC3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-120 amino acids from human Cleaved LC3A.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 14272 Da
• Gene Name: microtubule associated protein 1 light chain 3 alpha
• Database Link:
  NP_115903
  Entrez Gene 66734 MouseEntrez Gene 362245 RatEntrez Gene 84557 Human
  UniProt ID Q9H492
• Synonyms: ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3