KLF6 Rabbit Polyclonal Antibody

CAT#: TA324852

Rabbit polyclonal KLF6 Antibody (C-term)

Product Images

Western blot analysis of KLF6 Antibody (C-term) (Cat. #TA324852) in mouse stomach tissue lysates (35ug/lane). KLF6 (arrow) was detected using the purified Pab.

• 

  Formalin-fixed and paraffin-embedded human testis tissue reacted with KLF6 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

• 

  IF image of Hela cell stained with KLF6 Antibody (C-term) (Cat#TA324852).Hela cells were incubated with KLF6 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). KLF6 immunoreactivity is localized to Nucleus significantly.

• 

  Flow cytometric analysis of widr cells using KLF6 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:10~50, FC: 1:10~50, IF: 1:10~50
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This KLF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-186 amino acids from the C-terminal region of human KLF6.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 31865 Da
• Gene Name: Kruppel-like factor 6
• Database Link:
  NP_001291
  Entrez Gene 23849 MouseEntrez Gene 1316 Human
  UniProt ID Q99612
• Synonyms: BCD1; CBA1; COPEB; CPBP; GBF; PAC1; ST12; ZF9

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors