SPHK1 Rabbit Polyclonal Antibody

CAT#: TA324909

Rabbit polyclonal SPHK1 Antibody (Center)

Product Images

The anti-SPHK1 Pab (Cat. #TA324909) is used in Western blot to detect SPHK1 in mouse kidney tissue lysate.

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  Western blot analysis of anti-SPHK1 Antibody (Center) (Cat.#TA324909) in 293 cell line lysates (35ug/lane). SPHK1 (arrow) was detected using the purified Pab.

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  Western blot analysis of SPHK1 (arrow) using rabbit polyclonal SPHK1 Antibody (R301) (Cat.#TA324909). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1 gene.

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  Western blot analysis of lysates from 293, HepG2, HUVEC, Raji, rat C6, mouse C2C12 cell line (from left to right), using SPHK1 Antibody (R301) (Cat. #TA324909). TA324909 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:50~100
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human SPHK1.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42518 Da
• Gene Name: sphingosine kinase 1
• Database Link:
  NP_892010
  Entrez Gene 20698 MouseEntrez Gene 170897 RatEntrez Gene 710441 MonkeyEntrez Gene 8877 Human
  UniProt ID Q9NYA1
• Synonyms: SPHK

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Fc gamma R-mediated phagocytosis, Metabolic pathways, Sphingolipid metabolism, VEGF signaling pathway