Cytochrome P450 3A5 (CYP3A5) Rabbit Polyclonal Antibody

CAT#: TA324989

Rabbit polyclonal CYP3A5 Antibody (C-term)

Product Images

Western blot analysis of anti-CYP3A5 Antibody (C-term) (Cat.#TA324989) in 293 cell line lysates (35ug/lane). CYP3A5 (arrow) was detected using the purified Pab.

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  Western blot analysis of lysate from SK-BR-3 cell line, using CYP3A5 Antibody (C-term) (Cat. #TA324989). TA324989 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

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  Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CYP3A5 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  Confocal immunofluorescent analysis of CYP3A5 Antibody (C-term) (Cat#TA324989) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:10~50, IF: 1:10~50
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This CYP3A5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-502 amino acids from the C-terminal region of human CYP3A5.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57109 Da
• Gene Name: cytochrome P450 family 3 subfamily A member 5
• Database Link:
  NP_000768
  Entrez Gene 1577 Human
  UniProt ID P20815
• Synonyms: CP35; CYPIIIA5; P450PCN3; PCN3

Reference Data

• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Drug metabolism - cytochrome P450, Drug metabolism - other enzymes, Linoleic acid metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism