HSP90AB1 Mouse Monoclonal Antibody [Clone ID: 8D3]

CAT#: TA326370

Mouse monoclonal Hsp90 complex Antibody

Product Images

Hsp90 complex isolation (IP) from rabbit reticulocyte lysate (8D3), SDS PAGE.

Specifications

Product Data

• Clone Name: 8D3
• Applications: IP
• Reactivities: Human, Mouse, Rat, Rabbit. Not tested on other species
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Ah receptor (Aryl hydrocarbon receptor)
• Formulation: PBS, 50% glycerol, 0.09% sodium azide
• Concentration: lot specific
• Purification: PEG Purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: heat shock protein 90kDa alpha family class B member 1
• Database Link:
  NP_031381
  Entrez Gene 15516 MouseEntrez Gene 301252 RatEntrez Gene 3326 Human
  UniProt ID P08238
• Background: Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex . Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (12% of cytosolic protein). It carries out a number of housekeeping functions including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90- regulated proteins that have been discovered to date are involved in cell signaling . The number of proteins now know to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5 When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function .
• Synonyms: D6S182; HSP84; HSP90B; HSPC2; HSPCB
• Note: Immunoprecipitates 90kDa proteins corresponding to the molecular mass of Hsp90. Co-immunoprecipitates Hsp90 complexes, including Hsp70, Hop, Ah receptors, glucocorticoid receptors, heme-regulated eukaryotic initiation factor 2a (eIF-2a) kinase (HRI).

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Antigen processing and presentation, NOD-like receptor signaling pathway, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer