HSP90AB1 Rabbit Polyclonal Antibody [Clone ID: N/A]

CAT#: TA326372

Rabbit polyclonal Hsp90 Antibody

Product Images

Western blot analysis of Hsp90 -Lane 1-hsp90alpha-Lane 2-hsp90beta- showing Hsp90 specificity.

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  Western blot analysis of Hsp90 in various cell lines, using a 1:1000 dilution of the antibody

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  Hsp90 visualized using the antibody

Specifications

Product Data

• Clone Name: N/A
• Applications: IF, WB
• Recommended Dilution: WB: 1:20,000-40,000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Full length protein Hsp90
• Formulation: Rabbit antiserum
• Concentration: lot specific
• Purification: Rabbit antiserum
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: heat shock protein 90kDa alpha family class B member 1
• Database Link:
  NP_031381
  Entrez Gene 15516 MouseEntrez Gene 301252 RatEntrez Gene 3326 Human
  UniProt ID P08238
• Background: Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex . Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (12% of cytosolic protein). It carries out a number of housekeeping functions including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90-regulated proteins that have been discovered to date are involved in cell signaling . The number of proteins now know to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5. When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation.In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function .
• Synonyms: D6S182; HSP84; HSP90B; HSPC2; HSPCB
• Note: Detects ~90kDa proteins corresponding to the molecular mass of Hsp90a/β.

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Antigen processing and presentation, NOD-like receptor signaling pathway, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer