AKT2 Rabbit Polyclonal Antibody [Clone ID: N/A]

CAT#: TA326431

Rabbit polyclonal Akt2 (PKB beta) Antibody

Product Images

Western blot analysis of AKT2 (PKBbeta) in cell line mix using a 1:1000 dilution of the antibody

Specifications

Product Data

• Clone Name: N/A
• Applications: WB
• Recommended Dilution: 1:3000 dilution was sufficient for detection of PKBβ in 20ug of HeLa cell lysate by ECL immunoblot analysis.
• Reactivities: Bovine, Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Xenopus, Dog, Pig
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A five residue synthetic peptide based on the human Akt2, coupled to KLH
• Formulation: TBS, 50% glycerol, 0.09% sodium azide
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: AKT serine/threonine kinase 2
• Database Link:
  NP_001617
  Entrez Gene 11652 MouseEntrez Gene 25233 RatEntrez Gene 449021 DogEntrez Gene 700591 MonkeyEntrez Gene 208 Human
  UniProt ID P31751
• Background: Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 . Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target . Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration .
• Synonyms: HIHGHH; PKBB; PKBBETA; PRKBB; RAC-BETA
• Note: Detects a 65kDa protein, corresponding to the molecular mass of Akt2 PKBβ on SDS PAGE immunoblots. Does not react with PKBa (Akt1) or PKB? (Akt3).

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Insulin signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Melanoma, mTOR signaling pathway, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Tight junction, Toll-like receptor signaling pathway, VEGF signaling pathway