Piccolo (PCLO) Rabbit Polyclonal Antibody

CAT#: TA326449

Rabbit Polyclonal Anti-Piccolo Antibody

Product Images

Western blot analysis of Piccolo in mouse and rat brain lysate, using a 1:1000 dilution of the antibody

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  Piccolo BTX. Neuromuscular junction whole musle samples from adult mouse, with 1:400 dilution of the antibody, secondary (Alexa 488 goat anti-Rabbit from invitrogen). It shows bright staining of the NMJ very selectively as well as some terminals, very brig

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:1000
• Reactivities: Human, Mouse, Rat. Not yet tested on other species
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Full length protein
• Formulation: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Concentration: lot specific
• Purification: Protein G Purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: piccolo presynaptic cytomatrix protein
• Database Link:
  NP_055325
  Entrez Gene 26875 MouseEntrez Gene 56768 RatEntrez Gene 27445 Human
  UniProt ID Q9Y6V0
• Background: Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains . In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) . Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 .
• Synonyms: ACZ
• Note: Detects ~550kDa corresponding to the molecular weight of Picollo. Multiple isoforms can be detected. No cross-reactivity to Bassoon.