Prohibitin (PHB) Rabbit Polyclonal Antibody

CAT#: TA326760

PHB Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using PHB antibody.

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  Immunohistochemistry of paraffin-embedded rat spleen using PHB antibody at dilution of 1:200 (400x lens).

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  Immunohistochemistry of paraffin-embedded rat testis using PHB antibody at dilution of 1:200 (200x lens).

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  Immunofluorescence analysis of HeLa cell using PHB antibody. Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IF 1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human PHB
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30 kDa
• Gene Name: prohibitin
• Database Link:
  NP_002625
  Entrez Gene 18673 MouseEntrez Gene 25344 RatEntrez Gene 5245 Human
  UniProt ID P35232
• Background: The prohibitins, called PHB1 and PHB2, are highly conserved proteins that are present in multiple compartments in eukaryotic cells. PHB1 is 30kDa tumor suppressor protein involved in cell cycle control. PHB1 has been found in mitochondria, the nucleus and the plasma membrane, as well as extracellularly in circulation. In mitochondria prohibitins mainly exist as membrane-bound ring complexes and function as chaperones maintaining mitochondrial protein stability during protein synthesis and transportation. In the nucleus prohibitins interact with transcription factors such as Rb and p53 to regulate target gene transcription. Extracellular prohibitin can bind and activate C3 to enhance complement activation.
• Synonyms: HEL-215; HEL-S-54e; PHB1

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors