MLH1 Rabbit Polyclonal Antibody

CAT#: TA326801

Rabbit anti-MLH1 Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using MLH1 antibody.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB 1:500 - 1:2000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human MLH1
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 85 kDa
• Gene Name: mutL homolog 1
• Database Link:
  NP_000240
  Entrez Gene 4292 Human
  UniProt ID P40692
• Background: Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. MLH1 is the human homologue of the E. coli MMR gene mutL. MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting of MLH1 and PMS1 ((MutL-a), PMS2 (MutL-β) or MLH3 (MutL-?). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases and DNA ligase (reviewed in 1). Inactivation of the MLH1 gene causes genome instability and predisposition to cancer. The MLH1 gene is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). MLH1 also plays a role in meiotic recombination .
• Synonyms: COCA2; FCC2; hMLH1; HNPCC; HNPCC2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Colorectal cancer, Endometrial cancer, Mismatch repair, Pathways in cancer