DDX58 Rabbit Polyclonal Antibody

CAT#: TA326840

DDX58 Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using DDX58 antibody.

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  Immunohistochemistry of paraffin-embedded human rectal cancer using DDX58 antibody at dilution of 1:200 (200x lens).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50- 1:200
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide of human DDX58
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: DEXD/H-box helicase 58
• Database Link:
  NP_055129
  Entrez Gene 23586 Human
  UniProt ID O95786
• Background: Antiviral innate immunity depends on the combination of parallel pathways triggered by virus detecting proteins in the Toll-like receptor (TLR) family and RNA helicases, such as Rig-I (retinoic acid-inducible gene I) and MDA-5 (melanoma differentiation-associated antigen 5), which promote the transcription of type I interferons (IFN) and antiviral enzymes. TLRs and helicase proteins contain sites that recognize the molecular patterns of different virus types, including DNA, single-stranded RNA (ssRNA), double-stranded RNA (dsRNA), and glycoproteins. These antiviral proteins are found in different cell compartments; TLRs (i.e. TLR3, TLR7, TLR8, and TLR9) are expressed on endosomal membranes and helicases are localized to the cytoplasm. Rig-I expression is induced by retinoic acid, LPS, IFN, and viral infection. Both Rig-I and MDA-5 share a DExD/H-box helicase domain that detects viral dsRNA and two amino-terminal caspase recruitment domains (CARD) that are required for triggering downstream signaling. Rig-I binds both dsRNA and viral ssRNA that contains a 5-triphosphate end not seen in host RNA. Though structurally related, Rig-I and MDA-5 detect a distinct set of viruses. The CARD domain of the helicases, which is sufficient to generate signaling and IFN production, is recruited to the CARD domain of the MAVS/VISA/Cardif/IPS-1 mitochondrial protein, which triggers activation of NF-?B, TBK1/IKKe, and IRF-3/IRF-7.
• Synonyms: RIG-I; RIGI; RLR-1

Reference Data

• Protein Pathways: Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway