Integrin Linked Kinase (ILK) Rabbit Polyclonal Antibody

CAT#: TA326860

Rabbit anti-ILK Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using ILK antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500-1:1000;IHC: 1:50-1:100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human ILK
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: integrin linked kinase
• Database Link:
  NP_004508
  Entrez Gene 16202 MouseEntrez Gene 170922 RatEntrez Gene 3611 Human
  UniProt ID Q13418
• Background: Integrin-linked kinases (ILKs) couple integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility. ILK functions as a scaffold bridging the extracellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin. ILK phosphorylates Akt at Ser473, GSK-3 on Ser9, myosin light chain 2 (MLC2) on Ser18/Thr19, as well as affixin. These phosphorylation events are key regulatory steps in modulating the activities of the targets. ILK activity is stimulated by PI3 kinase and negatively regulated by the tumor suppressor PTEN and a PP2C protein phosphatase, ILKAP. It has been suggested that the conserved Ser343 residue in the activation loop plays a key role in the activation of ILK1.
• Synonyms: HEL-S-28; ILK-1; ILK-2; P59; p59ILK

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Endometrial cancer, Focal adhesion, PPAR signaling pathway