Fibrillarin (FBL) Rabbit Polyclonal Antibody

CAT#: TA326942

FBL Rabbit Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using Fibrillarin/U3 RNP antibody (TA326942) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50 - 1:200
• Reactivities: Human, Mouse, Monkey, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human FBL
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34 kDa
• Gene Name: fibrillarin
• Database Link:
  NP_001427
  Entrez Gene 14113 MouseEntrez Gene 292747 RatEntrez Gene 700286 MonkeyEntrez Gene 2091 Human
  UniProt ID P22087
• Background: Fibrillarin is a 2-O-methyltransferase located in fibrillar regions and Cajal bodies of the nucleolus, where RNA transcription and pre-RNA processing take place. Fibrillarin associates with several other structual proteins as well as box C/D snoRNA to form a complex that functions in pre-rRNA processing, pre-rRNA methylation and ribosome assembly. This complex catalyzes site-specific 2-O-ribose methylation of targeted nucleotides within the rRNA sequence. The sequence, structure and function of fibrillarin are highly conserved and fibrillarin gene expression is essential for early embryonic development.
• Synonyms: FIB; FLRN; RNU3IP1

Reference Data

• Protein Families: Stem cell - Pluripotency