NCF2 Rabbit Polyclonal Antibody

CAT#: TA326975

Rabbit anti-NCF2 Polyclonal Antibody

Product Images

Western blot analysis of THP1 cell and mouse spleen cell lysate using NCF2 antibody.

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  Immunohistochemistry of paraffin-embedded human esophageal cancer using NCF2 antibody at dilution of 1:100 (400x lens).

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  Immunohistochemistry of paraffin-embedded human esophagus using NCF2 antibody at dilution of 1:100 (400x lens).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50- 1:200
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human NCF2
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: neutrophil cytosolic factor 2
• Database Link:
  NP_000424
  Entrez Gene 17970 MouseEntrez Gene 4688 Human
  UniProt ID P19878
• Background: The phagocytic NADPH oxidase is a multiprotein enzyme that catalyzes the reduction of oxygen to superoxide in response to pathogenic invasion. The NADPH oxidase consists of 6 subunits, including the membrane-bound p91 phox and p22 phox heterodimers (also known as cytochrome b558), the cytosolic complex of p40phox, p47phox and p67phox, and the small GTPase Rac2. Activation of NADPH oxidase is initiated by cytosolic complex phosphorylation, which induces a conformational change that leads to the translocation of the cytosolic complex to the membrane and formation of an active enzyme with cytochrome b558. Defects in p47phox, often resulting from recombination between p47phox and a nearby homologous pseudogene, cause chronic granulomatous disease. Elevated oxidative stress due to increased myocardial NADPH oxidase activity may be a contributing factor in heart failure.p67phox appears to coordinate assembly of NAPDH oxidase as it associates with multiple subunits as well as the a subunit of heterotrimeric G proteins. Mutations in the corresponding p67phox gene are also associated with a form of autosomal recessive chronic granulomatous disease .
• Synonyms: NCF-2; NOXA2; P67-PHOX; P67PHOX

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Leukocyte transendothelial migration