PPP1A (PPP1CA) Rabbit Polyclonal Antibody

CAT#: TA327322

Rabbit anti-PPP1CA Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using PPP1CA antibody (TA327322) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500 - 1:2000;IHC 1:50- 1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human PPP1CA
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: protein phosphatase 1 catalytic subunit alpha
• Database Link:
  NP_002699
  Entrez Gene 19045 MouseEntrez Gene 24668 RatEntrez Gene 5499 Human
  UniProt ID P62136
• Background: Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is highly conserved in eukaryotic cells. Four isoforms of PP1 have been characterized:PP1a, PP1d, PP1?1 and PP1?2. Involvement in cell cycle regulation is one of the biological functions of PP1. It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis. A cell cycle-dependent phosphorylation at Thr320 of PP1a by cdc2 kinase inhibits PP1a activity.
• Synonyms: PP-1A; PP1A; PP1alpha; PPP1A

Reference Data

• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Focal adhesion, Insulin signaling pathway, Long-term potentiation, Oocyte meiosis, Regulation of actin cytoskeleton, Vascular smooth muscle contraction